Journal: Cell reports

Article Title: Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk

doi: 10.1016/j.celrep.2019.11.079

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant DNA HA-HIF1α P402A/P564A-pcDNA3 Yan et al., 2007 Addgene: 338299 pH IV-ODD-EGFP-IRES-dTomato Bagnall et al., 2014 Addgene: 395015 SUCLG2 DNASU HsCD00445557 CXCR1 Sino Biological HG10856-NF CCR2 Sino Biological HG16084-NF EF1a-GFP-IRES-Puro (pEGIP) Zou et al., 2009 Addgene: 26777 Software and Algorithms GraphPad Prism 7 GraphPad Software RRID: SCR_002798 FIJI [ImageJ] National Institutes of Health RRID:SCR_002285 Aperio ScanScope / Pixel Count v9 Leica Biosystems RRID: SCR_014311 Thermo Xcalibur ThermoFisher Scientific RRID: SCR_014593 MaxQuant Max Planck Institute RRID: SCR_014485 Perseus Max Planck Institute RRID: SCR_015753 Molecular Signatures Database Broad Institute, MIT RRID: SCR_016863 MetaboAnalyst McGill University RRID: SCR_015539 Other Invasion transwell insert (8μm pore) ThermoFisher Scientific 353097 Co-culture transwell insert (0.4μm pore) Thomas Scientific 3450 Collagen type 1 [rat tail] Corning 354236 PureLink HiPure Plasmid Maxiprep Kit ThermoFisher Scientific K2100-06 IDEXX CellCheck IDEXX Bioresearch https://www.idexxbioanalytics.com/cellcheck Open in a separate window KEY RESOURCES TABLE Western Immunoblotting Cell lysates were prepared in 125μL RIPA buffer (100mM Tris/HCl [pH 7.4], 150mM NaCl, 1mM EDTA, 0.1% (w/v) sodium dodecyl sulfate, 0.5% sodium deoxycholate, 1% Triton X-100 and supplemented with phosphatase/protease inhibitors immediately prior to use).

Techniques: Ex Vivo, Isolation, Microarray, Recombinant, Mutagenesis, Enzyme-linked Immunosorbent Assay, Software, Plasmid Preparation

Journal: Cell reports

Article Title: Recursive feedback between matrix dissipation and chemo-mechanical signaling drives oscillatory growth of cancer cell invadopodia

doi: 10.1016/j.celrep.2021.109047

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 280 kDa Matrigel Corning Cat# 354230 Calcium Sulfate (CaSO4) Sigma Aldrich Cat# 255696-10G Lipofectamine 3000 ThermoFisher Scientific Cat# L3000075 OptiMEM ThermoFisher Scientific Cat# 31985062 EGF Peprotech, Inc. Cat# AF-100-15 Puromycin ThermoFisher Scientific Cat# A1113803 Neomycin ThermoFisher Scientific Cat# 21810031 Fluoresceinamine Sigma Aldrich Cat# 20162 Critical commercial assays MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 GeneJet Plasmid Miniprep kit ThermoFisher Scientific Cat# K0701 Deposited data Measured invadopodia dynamics of MDA-MB-231 This paper https://doi.org/10.17632/tp7fkkxk44.2 Measured circularity of HT-1080 cells Wisdom et al., 2018 https://doi.org/10.17632/tp7fkkxk44.2 Experimental models: Cell lines MDA-MB-231 ATCC ATCC® HTB-26 HT-1080 cells ATCC ATCC® CCL-121 Recombinant DNA RFP-LifeAct Gift from A. Dunn N/A Software and algorithms Simulation of invadopodia dynamics This paper N/A High throughput invadopodia dynamics analysis Chang et al., 2020 N/A MATLAB Mathworks https://www.mathworks.com/products/matlab.html Imaris Oxford Instruments https://imaris.oxinst.com/ ImageJ ImageJ https://imagej.net/Welcome Other Stress-controlled rheometer TA Instruments AR2000EX Open in a separate window KEY RESOURCES TABLE A chemo-mechanical model for invadopodia dynamics in 3D matrices is developed Oscillations occur when timescales for extension and myosin dynamics are comparable Matrix plastic strain accumulates by cyclic ratcheting during invadopodia growth High matrix plasticity facilitates the oscillatory growth of invadopodia

Techniques: Blocking Assay, Recombinant, Modification, Plasmid Preparation, Software, High Throughput Screening Assay